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โครงการรวบรวมและจัดทําวารสารอิเล็กทรอนิกส์ มหาวิทยาลัยเกษตรศาสตร์

์
Thai J. For. 31 (1) : 10-19 (2012) วารสารวนศาสตร 31 (1) : 10-19 (2555)

Original article

Genetic Diversity of Cinnamomum porrectum (Roxb.) Kosterm.
in Southern Thailand Detected by
Inter Simple Sequence Repeat (ISSR) Analysis

Suwimon Uthairatsamee Damrong Pipatwattanakul

Faculty of Forestry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
E-mail: ampai44@hotmail.com

Received: April 26, 2011 Accepted: June 3, 2011

ABSTRACT
Genetic diversity of Cinnamomum porrectum (Roxb.) Kosterm. (‘Thep tharo’) in southern
Thailand was assessed by analyzing molecular genetic markers at the population level. Inter
simple sequence repeat (ISSR) markers were used to analyze the genetic diversity of six
populations in Phangnga, Phuket, Phatthalung, Satun, Krabi, and Songkhla provinces. In total,
73 amplified bands were obtained from 117 individuals of all sampled populations using seven
primers. At the species level, the percentage of polymorphic loci (P), the mean expected hetero
zygosity (H ), and the Shannon’s information index of diversity (I) were high (87.67%, 0.2883,
e
and 0.4343, respectively). However, P, H , and I were relatively low at the population level with
e
averages of 47.03%, 0.1689, and 0.2508, respectively. Among the six populations investigated, the
Songkhla and Phangnga populations revealed the highest genetic diversity, followed by Satun,
Phatthalung, Phuket and Krabi populations, respectively. Based on Nei’s genetic differentiation
(G ) value, 35.51% of genetic differentiation existed among populations. The gene flow (N )
st
m
was 0.9081. UPGMA cluster analysis divided the populations into two main groups.One
consisted of the populations of Satun, Songkhla, Phangnga, and Phatthalung, while the other
was composed of the Phuket and Krabi populations. Based on the results of the present study and
field survey, it could be suggested that in situ gene conservation is very important for this species,
especially in the Songkhla and Phangnga populations due to their higher genetic diversity and
larger population sizes. At the same time, ex situ gene conservation should be established as an
option to conserve the genetic material of the Satun, Phatthalung, Phuket, and Krabi populations
that showed low genetic diversity and small population sizes.

Keywords: Cinnamomum porrectum, ‘Thep tharo’, genetic diversity, ISSR, conservation

INTRODUCTION family Lauraceae. It is mostly distributed
throughout southern Thailand. All plant parts
Cinnamomum porrectum (Roxb.) of this species provide various benefits to local
Kosterm., local name ‘Thep tharo’, is a people including medicine, food, and wood
construction, as well as for uses in religious
medicinal and aromatic tree belonging to the
and cultural ceremonies (Chayamarit, 1997;