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โครงการรวบรวมและจัดทําวารสารอิเล็กทรอนิกส์ มหาวิทยาลัยเกษตรศาสตร์

วารสารวนศาสตร 31 (1) : 10-19 (2555) 13
์

RESULTS AND DISCUSSION At the population level, all populations had a
wide range of genetic variation. The value of
ISSR Polymorphism P per population ranged from 16.44 to 78.08
For the 117 individuals of C. porrectum %, with an average of 47.03 %. He ranged
from the six populations, seven primers from 0.0699 to 0.2681, with an average of
produced a total of 73 amplified bands, of
which 64 (87.67 %) were polymorphic and 0.1689, and I ranged from 0.1008 to 0.3947,
9 (12.33 %) were monomorphic. The bands with an average of 0.2508 (Table 3). Among
per primer ranged from 8 to 13, with an the six populations investigated, the Songkhla
average of 10.43 ± 1.90. The size of the and Phangnga populations revealed the highest
amplified bands ranged from 150 to 3,000 genetic diversity, followed by the Satun,
bp (Table 2). Each of 117 individuals Phatthalung, Phuket and Krabi populations,
presented a unique ISSR genotype, indicating
extensive genetic variation in the populations respectively.
studied. The value of Nei’s genetic
differentiation (G ) was 0.3551, indicating
st
Genetic Diversity and Differentiation that about 35.51 % of the genetic variation
At the species level, the percentage was among populations. The level of gene
of polymorphic loci (P), the mean expected flow (N ), which was the number of migrating
heterozygosity (H ), and Shannon’s m
e individuals among populations per generation,
information index of diversity (I) were was calculated to be 0.9081 (Table 3).
87.67 %, 0.2883, and 0.4343, respectively.

Table 3 Genetic diversity parameters of Cinnamomum porrectum from six populations
based on inter simple sequence repeat (ISSR) markers.

Notes: P = percentage of polymorphic loci; H = the mean expected heterozygosity
e
(Nei, 1973); I = Shannon’s information index (Lewontin, 1972); G = Nei’s
st
genetic differentiation; N = Gene fl ow
m
According to the remaining populations the genetic diversity of C. porrectum at the
of C. porrectum in southern Thailand, which species level was relatively high (H = 0.2883
e
are rather small and fragmented, it could be and I = 0.4343) when compared with other
predicted that they should have be low species in the same genus also using ISSR
genetic diversity. However, the results for markers, such as C. osmophloeum (H =
e
the ISSR marker in this study showed that 0.2213 and I = 0.5746), C. macrostemon