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โครงการรวบรวมและจัดทําวารสารอิเล็กทรอนิกส์ มหาวิทยาลัยเกษตรศาสตร์

12 Thai J. For. 31 (1) : 10-19 (2012)

at 94 ˚C, followed by 30 s at 94 ˚C, 45 equilibrium at these ISSR marker loci. The
s annealing temperature of each primer following indices were used to quantify the
(Table 2), and 2 min extension at 72˚C for amount of genetic diversity within each
29 cycles, and 7 min at 72 ˚C for a final population examined: the percentage of
polymorphic loci (P), the mean expected
extension. The amplification products were heterozygosity (H ) (Nei, 1973), and Shannon’s
e
analyzed on 1.5% agarose gels in 1 × TBE information index of diversity (I). Genetic
buffer and detected by staining with ethidium diversity parameters (P, H , and I) were also
e
bromide. Band size was estimated from a calculated at the species level.
100 bp DNA ladder (Fermentas). Genetic differentiation among
populations was estimated by Nei’s gene
diversity statistic (Nei, 1973). The amount
Data Analysis of gene flow among these populations was
ISSR bands were used to assign loci estimated as Nm = 0.5(1-G )/G (McDermott
for each primer and scored as presence (1) and and McDonald, 1993). The pairwise genetic
st
st
absence (0). The band presence or absence distance between populations (Nei, 1972)
data matrix was analyzed by POPGENE was calculated to construct a UPGMA
(Yeh et al., 1999), making the assumption dendrogram.
that the populations are in Hardy-Weinberg

Table 1 Population codes and number of samples of Cinnamomum porrectum used
for inter simple sequence repeat (ISSR) analysis.
for inter simple sequence repeat (ISSR) analysis.

Ta
Table 2 List of selected inter simple sequence repeat (ISSR) primers, sequence, ble 2 List of selected inter simple sequence repeat (ISSR) primers, sequence,
annealing temperature, approximate size range (in base pairs) of the bands, annealing temperature approximate size range (in base pairs) of the bands
number of monomorphic and polymorphic bands.

Total 73.00 9.00 64.00
Average 10.43 1.29 9.14 87.67
SD 1.90 0.76 1.57
Note: % PPB = percentage of polymorphic loci