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โครงการหนังสืออิเล็กทรอนิกส์ด้านการเกษตร เฉลิมพระเกียรติพระบาทสมเด็จพระเจ้าอยู่หัว

การศึกษาศักยภาพของแอคติโนมัยสีทที่แยกได้จากพื้นที่ได้รับผลกระทบจากการ

เปลี่ยนแปลงสภาพภูมิอากาศบริเวณลุ่มน้ำปาย
Potential of actinomycetes isolated from areas affected

by climate change in Pai river basin
อมรรัตน์ ใจยะเสน จิตรา เกาะแก้ว อำนาจ เอี่ยมวิจารณ์
Amornrat Chaiyasen Jitra Kokaew Amnat Eamvijarn

กลุ่มวิจัยปฐพีวิทยา กองวิจัยพัฒนาปัจจัยการผลิตทางการเกษตร

ABSTRACT
The potential of actinomycetes isolated from Pai river basin, Mae Hong Son Province that
affected by different temperatures between summer and winter, was studied in 4 areas: 1) downstream
forest 2) downstream agricultural area 3) upstream forest and 4) upstream agricultural area in three
seasons: winter (December), summer (April) and rainy (July). Plant growth promoting properties including
siderophore production, phosphate solubilization and indole acetic acid (IAA) production were tested.
The results showed that Streptomyces sp. WF 4-1 has the highest siderophore production, while
Streptomyces sp. WF 10-1 has the highest phosphate solubility. Actinomycetes isolated from soil in

winter, summer and rainy season with the production of indole acetic acid were 42, 32 and 29 isolates,
respectively. Ten actinomycete isolates with highest plant growth promoting activity were selected to
test against plant pathogens. It was found that Streptomyces sp. WF 4-1 was highest effective in inhibiting
bacterial plant pathogens including Erwinia carotovora pv. carotovora, Ralstonia solanacearum,
Xanthomonas axonopodis pv. glycines and X. oryzae pv. oryzae. Streptomyces sp. WA 20-3 was the
most effective isolate for inhibiting X. campestris pv. campestris. In vitro growth inhibition of fungal
plant pathogens including Colletotrichum sp., Fusarium sp., Phytophthora palmivora, Rhizoctonia
solani, Rhizoctonia sp. and Sclerotium rolfsii showed that Streptomyces sp. RF 23-1 was effective in
fungal growth inhibition of all tested fungal plant pathogens. The result of non-antagonistic with
rhizobium showed that Streptomyces sp. WF 3-1, WF 4-1, WF 10-1, RF 12-4 and RF 23-1 were non-
antagonistic to three rhizobium species: Bradyrhizobium japonicum DASA 02006, B. liaoningense DASA
03018 and B. daqingense DASA 03084. Therefore, these actinomycetes can be used with the use of
those three rhizobium species. In greenhouse experiment, mungbean seedlings were found to be rotten
when planted with plant pathogenic fungi: Sclerotium rolfsii, even with Streptomyces sp. WF 4-1. In

addition, seedlings that inoculated with Streptomyces sp. RF 12- 4 was showed moderate disease
occurrence at level 2. Erwinia carotovora pv. carotovora caused mungbean seedlings to the mild
disease at level 1 but plant height, fresh weight, root length and root number among all treatments
was no statistical difference.
Keywords : Actinomycetes, Antagonist, Pai river basin, plant pathogen

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